First Advisor

Kuhn, Darl

Second Advisor

Mason, Robert T.

Third Advisor

Barnes, Stephen D.

College

College for Professional Studies

Degree Name

MS Database Technologies

School

School of Computer & Information Science

Document Type

Thesis - Open Access

Number of Pages

55 pages

Abstract

Shotgun proteomics refers to the direct analysis of complex protein mixtures to create a profile of the proteins present in the cell. These profiles can be used to study the underlying biological basis for cancer development. Closely studying the profiles as the cancer proliferates reveals the molecular interactions in the cell. They provide clues to researchers on potential drug targets to treat the disease. A little more than a decade old, shotgun proteomics is a relatively new form of discovery, one that is data intensive and requires complex data analysis. Early studies indicated a gap between the ability to analyze biological samples with a mass spectrometer and the information systems available to process and analyze this data. This thesis reflects on an automated proteomic information system at the University of Colorado Central Analytical Facility. Investigators there are using cutting edge proteomic techniques to analyze melanoma cell lines responsible for skin cancer in patients. The paper will provide insight on key design processes in the development of an Oracle relational database and automation system to support high-throughput shotgun proteomics in the facility. It will also discuss significant contributions, technologies, software, a data standard, and leaders in the field developing solutions and products in proteomics.

Date of Award

Fall 2011

Location (Creation)

Denver, Colorado

Rights Statement

All content in this Collection is owned by and subject to the exclusive control of Regis University and the authors of the materials. It is available only for research purposes and may not be used in violation of copyright laws or for unlawful purposes. The materials may not be downloaded in whole or in part without permission of the copyright holder or as otherwise authorized in the “fair use” standards of the U.S. copyright laws and regulations.

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